Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry

Laurence Poirier (1) , Jean-Claude Amiard (1) , Florence Mondeguer (2) , Françoise Quiniou (3) , Nicolas Ruiz (1) , Monique Montagu (1) , Yves François Pouchus (1)

1 Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques
2 EMH - Unité Écologie et Modèles pour l'Halieutique
3 BE - Unité Biogéochimie et Ecotoxicologie

Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.

Mots clés: Fungal peptide metabolites; Trichoderma sp.; Marine fungal contamination; Electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS); Matrix matched calibration

Domaines: Chimie analytique; Sciences de l'environnement

Laurence Poirier, Jean-Claude Amiard, Florence Mondeguer, Françoise Quiniou, Nicolas Ruiz, et al.. Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry. Journal of Chromatography A, 2007, 1160 (1-2), pp.106-113. ⟨10.1016/j.chroma.2007.04.006⟩. ⟨hal-03596314⟩