Food allergy enhances allergic asthma in mice

Tiphaine Bihouée (1, 2, 3) , Grégory Bouchaud (1, 2) , Julie Chesné (1, 2) , David Lair (1, 2) , Camille Rolland-Debord (1, 2) , Faouzi Braza (1, 2) , Marie-Aude Cheminant (1, 2) , Philippe Aubert (1) , Guillaume Mahay (1, 2) , Christine Sagan (1, 4) , Michel Neunlist (2, 5) , Sophie Brouard (6) , Marie Bodinier (7) , Antoine Magnan (1, 8, 2)

1 ITX - ITX - unité de recherche de l'institut du thorax
2 DHU 2020 - Médecine Personnalisée des Maladies Chroniques [CHU Nantes]
3 Département de pédiatrie [CHU Nantes]
4 Laboratoire d'anatomie et cytologie pathologiques [CHU Nantes]
5 Neuropathies du système nerveux entérique et pathologies digestives : implication des cellules gliales entériques
6 U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie
7 BIA - Unité de recherche sur les Biopolymères, Interactions Assemblages
8 Service de pneumologie [Nantes]

BACKGROUND: Atopic march refers to the typical transition from a food allergy in early childhood to allergic asthma in older children and adults. However the precise interplay of events involving gut, skin and pulmonary inflammation in this process is not completely understood. OBJECTIVES: To develop a mouse model of mixed food and respiratory allergy mimicking the atopic march and better understand the impact of food allergies on asthma. METHODS: Food allergy to ovalbumin (OVA) was induced through intra-peritoneal sensitization and intra-gastric challenge, and/or a respiratory allergy to house dust mite (HDM) was obtained through percutaneous sensitization and intra-nasal challenges with dermatophagoides farinae (Der f) extract. Digestive, respiratory and systemic parameters were analyzed. RESULTS: OVA-mediated gut allergy was associated with an increase in jejunum permeability, and a worsening of Der f-induced asthma with stronger airway hyperresponsiveness and pulmonary cell infiltration, notably eosinophils. There was overproduction of the pro-eosinophil chemokine RANTES in broncho-alveolar lavages associated with an enhanced Th2 cytokine secretion and increased total and Der f-specific IgE when the two allergies were present. Both AHR and lung inflammation increased after a second pulmonary challenge. CONCLUSION: Gut sensitization to OVA amplifies Der f-induced asthma in mice.

Mots clés: Allergy; Th2 cytokines; Gut; House dust mite; Immunity; Asthma

Mots clés: Mouse; Ovalbumin

Domaines: Médecine humaine et pathologie

Tiphaine Bihouée, Grégory Bouchaud, Julie Chesné, David Lair, Camille Rolland-Debord, et al.. Food allergy enhances allergic asthma in mice. Respiratory Research, 2014, 15 (1), pp.142. ⟨10.1186/s12931-014-0142-x⟩. ⟨inserm-02163519⟩